The quality of the microarray data was validated using qPCR carried out according to the manufacturer’s instructions (Applied Biosystems, Foster City, CA) on an ABI PRISM 7900 Sequence Detection System, using expression of RpL32 as a standard to normalize sample concentrations. All qPCR validation experiments utilized a time-course set of RNA samples from air- and ethanol-exposed flies that were derived independently from those used for microarray analysis. The relative transcript expression levels for genes in ethanol- and air-treated flies were determined by comparing to untreated controls. Taqman probesets (Applied Biosystems) used in this study were aay: Dm01822491_s1, AcCoAS: Dm01798031_g1, Akap200: Dm01803695_g1, aru: Dm01814168_g1, DnaJ-H: Dm01790940_g1, elk: Dm01842361_m1, for: Dm01808235_g1, jhamt: Dm01791790_g1, Idgf1: Dm01842859_g1, lush: Dm01823120_ g1, puc: Dm02135504_m1, RpL32: Dm02151827_g1, Sir2: Dm01844783_g1, Tdc1: Dm01815580_m1, Tsp42Eg: Dm01817973_ g1, Tps1: Dm01801466_g1, wdb: Dm02145441_g1. Gene data are from Flybase (http://www.flybase.org) release FB2009_05, as of May 29, 2009.
