Unexpectedly, glutamatergic neurons were found, although the caudate is known not to contain cell bodies of excitatory neurons. Their presence could reflect inadvertent inclusion of another brain region at the time of dissection; therefore, twenty samples that contained >10% of glutamatergic neurons were removed from all subsequent analyses (Supplementary Tables 1, 2), leaving 143 samples (74 with AUD, 69 without; 115 male, 28 female) with gene expression data for 1,121,762 nuclei, 250,537 of which also had ATAC data. There was no significant difference in relative abundance of cell types between samples from individuals with and without AUD (Extended Data Figure 1).
