Using Barrier 2.2 software, we also correlated geographic and genetic distances as measured by pair-wise Fst and great-circle coordinates of capitals or the city where an individual population sample had been recruited, respectively. The results overlapped with previous findings in that the first barrier was seen between Finland and all other samples, a second barrier separated Southern Italy from the remainder, a third was found between Western Russia, Poland and Lithuania on the one hand, and Bulgaria on the other, a fourth was seen between Kuusamo and Helsinki, and a fifth was between the Baltic region and Poland on the one hand, and Sweden on the other (Figure S4).
