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Chunk #8 — Methods — Single-cell RNA sequencing datasets

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Comprehensive analyses of RNA-seq and genome-wide data point to enrichment of neuronal cell type subsets in neuropsychiatric disorders.
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All cell type enrichment analyses were conducted using the KI dataset [8, 16–18] and the 10x Genomics dataset [19]. These datasets were selected because they cover brain regions that are generally accepted to be involved in the pathogenesis of brain-related disorders [20]. Additionally, their high coverage may enable the identification of different cell types. Detailed information about the 10x Genomics dataset, quality control, necessity of a randomized representative subset of cells, and cell type identification are reported in the Supplementary Methods. The quality control of the KI dataset is described elsewhere [16].