To complete our assessment of tumor heterogeneity via UDT-Seq, we further analyzed the sequences derived from the ovarian adenocarcinoma and small intestine sarcoma xenografts and their matched germline DNA. The ovarian xenograft shows a homozygous somatic mutation at TP53-R248Q, the most common inactivating mutation in TP53 (Table 1), a gene mutated in 96% of ovarian cancers [19]. We did not identify any mutations in the sarcoma xenograft sample. These additional results confirm that UDT-Seq can identify known and novel mutations in previously uncharacterized samples.
