(NICD), an intracellular effector of Notch signaling and a catalytic product of PSEN1 [31], was significantly decreased in AD-NPCs on day 28 of differentiation (Supplementary Figure 4b). In line with this finding, the transcript levels of Notch direct targets (HES1 and HEY1) were significantly lower in differentiating AD-NPCs. In contrast, the level of proneural factor NEUROG2, which is transcriptionally repressed by HES1/HEY1 [32], was markedly higher in AD-NPCs than in N-NPCs during differentiation (Figure 6g).
