Total cellular RNA was extracted from ∼5 × 106 cells using the RNA-BEE (QIAGEN), according to the manufacturer’s instructions, and reverse transcribed using the high-capacity cDNA synthesis kit from AB Biosystems. qPCR was done using SYBR green (Life Technologies). qPCR results were analyzed using SDS Software v 3.2 for 7900HT real-time PCR system. Primer sequences used are described in the Supplemental Experimental Procedures.
