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Chunk #9 — Material and Methods — Genotyping

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Genetic variation in GABRA2 moderates peer influence on externalizing behavior in adolescents.
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Genotype data were available for three SNPs (rs279826, rs279858, and rs279827) in strong linkage disequilibrium that capture the information of a long haplotype block of 109 kb where previous associations have been reported with alcohol dependence and impulsive-related traits. We report results for SNP rs279826 (intron 4) for which we have more genotyping data and similar results were found with the other two SNPs. Testing haplotypes produced similar results (data not shown). Details of the genotyping method are reported elsewhere (Villafuerte et al. 2012). Genotype frequencies for rs279826 are in Hardy–Weinberg equilibrium: AA 27.4% (N = 67), AG 48.8% (N = 119), and GG 23.8% (N = 58).