Chunk #32 — Materials and Methods — Worm Husbandry

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Lipid environment modulates the development of acute tolerance to ethanol in Caenorhabditis elegans.

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C. elegans strains were maintained as described [37]. Strains used in this study were: N2 (var. Bristol), AX201 npr-1(ky13), BZ613 ctbp-1(eg613) (4×outcrossed), RB733 ctbp-1(ok498), BZ846 ctbp-1(ok498); npr-1(ky13), MT8987 pag-3(n3098), JCB40 pag-3(n3098); npr-1(ky13), JCB25 pag-3(bet16); npr-1(ky13) (1×outcrossed), VC226 ida-1(ok409), RB2287 lips-7(ok3110), VC199 sir-2.1(ok434), RB1716 nhr-49(ok2165), VC870 nhr-49(gk405), VC837 bbs-1(ok1111), RB1600 tub-1(ok1972), BX110 fat-7(wa36); fat-5(tm420), CE541 sbp-1(ep79), BZ142 slo-1(eg142), JCB95 slo-1(ky389) (1×outcrossed), JCB96 lips-7(ok3110); slo-1(ky389), JCB97 slo-1(ky399) (1×outcrossed), and JCB98 lips-7(ok3110); slo-1(ky399).