These criteria allow for the clear identification of direct ethanol targets. However, the low-affinity and transient molecular interactions of ethanol make fulfillment of all four criteria challenging. Thus, some of the molecular targets we discuss will be referred to as “putative” direct targets to indicate only partial fulfillment of the preceding criteria. Targets that do not meet any of the criteria above or that do not have any molecular structures indicative of an ethanol-binding site are referred to as indirect targets.
