Chunk #8 — 2. Material and methods — 2.1. Passaging and maintenance of NPCs — 2.1.1. Thawing NPCs

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Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

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Immediately prior to thawing NPCs, the Matrigel–DMEM mixture was aspirated and replaced with 2 ml of pre-warmed NPC medium per well (Table 1).