5% DNA methylation causing significant changes in the expression of other genes, such as RASSF1, in the human brain [66], AMOTL2 in the human heart [67], and PGC1α in human muscles [68]. Therefore, although the detected DNA methylation changes in this current study are not considerably high (they varied between 2 to 15%), they were statistically significant for average DNA methylation (within R1, R3 and R5) during NSC differentiation, and for several specific CpG dinucleotides subsequent to decitabine treatment (within R2, R4, R5, and R6), and are likely to be biologically important.
