(Kornberg and Stryer 1988). Third, a broad class of regulatory factors, the ATP-dependent nucleosome remodelers, plays a key role in assembling, displacing, and evicting nucleosomes (Clapier and Cairns 2009). Finally, additional structural elements such as Heterochromatin Protein 1 (HP1) and Histone 1 (H1) can assemble multinucleosomal units with constrained structures. The combination of these forces defines both the location of nucleosome-depleted regions and boundaries, as well as the pressure to pack the remaining region, leading to the repeating template structure.
