RNA from a separate dissection of aPFC cortical tissue from all drug abuse and control cases was DNase-treated (Qiagen Inc., Valencia, CA) and evaluated using the Bioanalyzer. As previously described [14], RNA was reverse transcribed using AMV reverse transcriptase (Roche Molecular Biologicals, Indianapolis, IN). A commercial 18S RNA primer kit (Ambion, Austin, TX) was used to quantify the amount of cDNA examined. Primers were designed using MacVector software (Accelrys, San Diego, CA), and synthesized by Gene Probe Technology (Gaithersburg, MD): APOL2(F): aac cgc cac gat aaa gac cag; APOL2(B): cac cca caa act cct tca tca cc; CALM2(F): gca gaa tcc cac aga agc aga g, CALM2(B): gcc att gcc atc ctt atc aaa; SEMA3B(F): aga ctt tca gcc tgg agc gaa c, SEMA3B(B): gca aat ggg tgc ggt tgt ag.
