Using microfluidics and human iN cell technology, we have created a high-throughput screening platform and culturing device for simplified two-way neuronal circuit studies using human stem cell derived neurons. Within the platform, human iN cells form functional circuits which can be studied using calcium imaging and morphological analyses in high-throughput imaging systems such as the IN Cell Analyzer 6000. The impact of small molecules on neurocircuitry function can be efficiently probed in a parallelized manner using automated liquid handling and confocal microscopy. Thus, we have provided a proof-of-concept device and approach that can be further developed into circuitry-based models for studying human neuropsychiatric disorders. We hope that this platform will advance therapeutic developments targeting neuronal circuit function and provide a conceptual and technical framework for furthering novel cell-based assays and platforms in the treatment of neuropsychiatric disorders.
