Totally, 27 children with ASD were recruited from the Children Development and Behavioral Research Center of Harbin Medical University, and 41 TDs were recruited from the local kindergartens. None of them had genetic, neurological, or other psychiatric disorders, and none of the children were taking psychotropic medications. Written informed consent was obtained from the guardians of each subject prior to examination. This study protocol was approved by the ethics review committee of Harbin Medical University (Approval number: 2013004).