Finally, Table 2 (data sets 1 to 9) presents the relative coverage of the previously described motifs, although not all are present in each sample (the G|C ≥ 80% motif is absent in all of the microbes, and the set of bad promoters was only defined for the human genome). We note that the single statistic of relative coverage for the GC ≥ 85% motif provided a suitable assay for bias on R. sphaeroides, with Pacific Biosciences scoring 0.87 (best), Illumina 0.60 and Ion Torrent 0.10 (worst), while GC ≥ 75% did not clearly distinguish between Illumina and Pacific Biosciences data. The GC ≤ 10% motif was similarly useful for P. falciparum, with Pacific Biosciences scoring 0.89 (best), Illumina 0.58, and Ion Torrent 0.39 (worst). For these data, the (AT)15 motif also stood out, with Pacific Biosciences at 0.85, Illumina at 0.43, and Ion Torrent at 0.11. Importantly, just these few statistics provided a meaningful readout on the performance of the different technologies.
