We compared the transcriptome of each of our 45 organoid samples to each of the 524 RNAseq sampels of developmental transcriptome of the human brain from the BrainSpan Atlas (www.brainspan.org), to classify our samples as corresponding to a particular brain region and developmental age. We computed the Spearman correlation matrix between the log transformed expression levels of our samples and each of the BrainSpan sampes. Each iPSC-derived neuronal sample was classified as corresponding to a particular brain sample if its correlation coefficient was within the 95% confidence interval of the maximum correlation coefficient. Average maximum correlation value is 0.86.
