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Chunk #14 — General Methods — Experimental Design

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Adolescent but not adult rats exhibit ethanol-mediated appetitive second-order conditioning.
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Across procedures and to avoid overrepresentation of litters within each specific group, no more than two animals per litter (one male and one female) were assigned to any particular experimental condition. Each conditioning group was composed of approximately 5 males and 5 females. For blood and brain ethanol measurements, each experimental condition had an average of 6 males and 6 females.