Chunk #1 — Materials and Methods — Cell culture and reagents

Source

The opposite effects of acute and chronic alcohol on lipopolysaccharide-induced inflammation are linked to IRAK-M in human monocytes.

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RAW 264.7 macrophages were purchased from American Type Culture Collection and maintained in Dulbecco’s modified medium (Invitrogen Life Technologies) containing 10% FBS (HyClone). PD98059 and 4-methylpyrazole and cyanamide were purchased from Sigma-Aldrich. LPS (Echerichia coli strain 0111:B4) was from Difco and NFκB oligo synthesized by Oligos.