Whole-cell patch-clamp recordings were performed from cells cocultured with astrocytes after 6 weeks of differentiation. The bath was constantly perfused with artificial cerebrospinal fluid (ACSF) (115 mM NaCl, 2 mM KCl, 3 mM CaCl2, 10 mM glucose, and 1.5 mM MgCl2). The recording micropipettes (tip resistance 3–6 MΩ) were filled with internal solution (140 mM K-gluconate, 5 mM KCl, 2 mM MgCl2, 0.2 mM EGTA, 2.5 mM Na-ATP, 0.5 mM Na-GTP, and 10 mM Na2-phosphocreatine). Additional details are provided in the Supplemental Experimental Procedures. Statistical comparisons of wild-type and SZCD groups were made using the nonparametric Kolmogorov-Smirnov two-tailed test, with a significance criterion of p = 0.05.
