Candidate polymorphisms for the CHRNA6 and CHRNB3 genes were identified using the SNPbrowser Software version 3.5 from Applied Biosystems (http://www.appliedbiosystems.com), the public database, dbSNP (http://www.ncbi.nlm.nih.gov/SNP/) and other published research. SNPs were chosen to span the two genes, have moderate minor allele frequencies, and assay reliably. The majority of SNPs chosen were identical to those used in Zeiger et al except for a few additional SNPs added to examine regions where association had recently been reported. The structures of the CHRNA6 and CHRNB3 genes, and the SNPs selected, are shown in Figure 1, with SNPs examined in this study shown in bold. Other recently published SNPs are also included in grey to show proximity and spacing.
