Here, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$Y^{\prime}$$\end{document}Y′ is the relative abundance of cell counts. The additional condition on the reference cell type occurring in almost every sample is necessary to prevent very rare cell types from being selected, where small random changes in cell counts have a large impact on the relative abundance. Therefore, we recommend setting \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$t=0.95$$\end{document}t=0.95, meaning that the reference cell type has to be present in at least 95% of samples. If no such cell type exists, this constraint can be relaxed by lowering t.
