On the other hand, consistent with RT-PCR and qRT-PCR analysis, EtOH caused a shift in Mcl-1L / Mcl-1S ratio by favoring Mcl-1S splicing over Mcl-1L (Fig. 6e). Interestingly, when the true copies of each isoform transcripts were determined and compared by ddPCR, the influence of EtOH on induction of Mcl-1S and reduction of Mcl-1L isoform splicing was even more dramatic than what it was suggested by RT-PCR and qRT-PCR analysis (compare Fig. 5 and Fig. 6).
