membrane slides (Leica Microsystems). Any given anatomical structure was first identified on the basis of histological data, and then sampled in a series of contiguous coronal slabs in both hemispheres. RNA was isolated from each sample and used to generate labelled cRNA probes for hybridization to custom 64K Agilent microarrays. The output of this pipeline was a set of microarrays that sample the entire spatial extent of neocortical gyri that could be reproducibly identified across individuals, as well as subcortical nuclear structures, at the resolution allowed by Nissl staining and sample size requirements for microarray analysis. One-hundred and seventy distinct structures were assayed at least once in both brains, and 146 structures twice or more (Supplementary Table 2). Sample locations were mapped back into the native brain MRI coordinates and subsequently to Montreal Neurological Institute (MNI) coordinate space7.
