To further evaluate the impact of oxidative stress on PV interneurons, we quantified parvalbumin-IR (PV-IR). Overall PV-IR intensity was significantly reduced in mPFC and S1 cortex of KO mice after 5 weeks of PWSI, but not in fGluN1 controls (Figure 4). Unlike PV, fluorescence immunoreactivity intensity for calretinin (Figure S3A, C in Supplement 1) and calbindin (Figure S3B, C in Supplement 1), another calcium binding proteins, did not differ between experimental groups. Interestingly, reduction of PV-IR upon PWSI in KO mice was prevented by chronic treatment of apocynin (Figure 4), suggesting that reduced PV expression in PWSI KO mice was associated with elevated oxidative stress in PV neurons.
