PCR cycles were as follows: 95°C for 10 min, followed by 40 cycles of 95°C for 15 sec and 60°C for 60 sec. A standard curve consisting of a 4-level serial dilution of 100%, 50%, 25%, and 12.5% cDNA from 20-week old neural cells differentiated from one subject was added to each plate to determine the relative mRNA expression among all subjects and across different qPCR plates. Data are displayed as mRNA abundance normalized to the housekeeping gene GUSB where a unit of 1 is equivalent to the abundance of the target gene relative to GUSB in the reference RNA sample.
