the length of the neurites continued to steadily increase throughout the 15 days of the differentiation protocol. However, the length of the neuritis did not increase significantly after D3, as no significant differences in neurite lengths were detected between neurons when assessed on D6, D9, D12, and D15 despite a trend toward an increase throughout the differentiation days. Overall, we found that the neurites of cultured neurons grow in length from D3 to D9; however, when assessed on later development days, both the area of the soma and the length of neurites decreased (Fig. 3).
