Percent specific binding was 94% (S.D. ± 1.35) averaged across films, documenting high sensitivity of binding and autoradiographic methods employed here. Social isolation increased cannabinoid receptor densities in the rostral dorsomedial (Rearing main effect: F1,28 = 4.24, P = 0.049; η2 = 0.119; Fig. 2a) and ventrolateral caudate putamen (Rearing main effect: F1,28 = 4.21, P = 0.05; η2 = 0.127; Fig. 2b) compared to group rearing (see also Fig. 2c–f). However, social isolation decreased cannabinoid receptor densities in the supraoptic hypothalamic nucleus (Rearing main effect: F1,21 = 5.89, P = 0.024; η2 = 0.194; Fig. 3a) and ventrolateral thalamic nuclei (Rearing main effect: F1,24 = 4.56, P = 0.043; η2 = 0.154; Fig. 3b) compared to group rearing (see also Fig. 4–5).
