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Chunk #47 — PROCEDURE — 3′ A-tailing of blunt-ended DNA library TIMING 1 h — Bioinformatics analysis: quality metrics and basic mutation analysis TIMING variable

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Detecting ultralow-frequency mutations by Duplex Sequencing.
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CRITICAL The processed DCS .bam files can be used in any downstream analysis that is desired. We present the basic data analysis protocol that we currently use.