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Chunk #85 — METHODS — Antibody Selection

Source
lincRNAs act in the circuitry controlling pluripotency and differentiation.
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yes

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We selected chromatin proteins that have been implicated in regulation of the pluripotent state along with their known associated ‘reader’, ‘writer’, and ‘eraser’ complexes. Specifically, we tested antibodies against 40 chromatin proteins, corresponding to 28 chromatin complexes. In many cases, we tested multiple antibodies against the same target protein to try and identify an antibody that worked well for immunoprecipitation. A full list of tested complexes and their associated antibodies are listed in Supplemental Table 18.