Accompanied with the premature neuronal differentiation, the cell growth rate of AD-NPCs was significantly lower than that of N-NPCs during this process (Figure 2e). Moreover, significantly lower percentage of BrdU+ proliferative cells was detected in differentiating AD-NPCs compared to those in control cells on day 28 of differentiation (Figure 2f and 2g), in accordance with lower percentage of Nestin+ NPCs and less number of Nestin+ cells in AD-NPCs during differentiation. Furthermore, higher percentage of TUNEL+ apoptotic cells was observed in differentiating AD-NPCs on day 28 (Figure 2h and 2i).
