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Chunk #10 — Materials and Methods

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Stress-response pathways are altered in the hippocampus of chronic alcoholics.
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We collected lists of differentially expressed genes from 10 other gene expression studies of post mortem brain tissue comparing alcoholics to controls (Flatscher-Bader et al., 2010; Flatscher-Bader et al., 2005; Iwamoto et al., 2004; Kryger & Wilce, 2010; Lewohl et al., 2000; Liu et al., 2007; Liu et al., 2006; Mayfield et al., 2002; Sokolov et al., 2003; Zhou et al., 2011b). Similarly, we assembled lists of genes identified in 12 recent GWAS studies of risk for alcoholism or related traits (Bierut et al., 2010; Edenberg et al., 2010; Foroud et al., 2007; Hack et al., 2011; Johnson et al., 2011; Kendler et al., 2011; Lind et al., 2010; Treutlein et al., 2009; Wang et al., 2012; Xuei et al., 2006; Zlojutro et al., 2011; Zuo et al., 2012). We annotated the list of differentially expressed genes from our study (Supplemental Table S2) to show these overlaps. We also created a list of genes identified by 2 or more studies (including the present one) in Supplemental Table S4; these will be referred to as “multiply-identified genes” in the rest of the text.