bp of the implicated DMR. In total, 1578 human CpGs were located within the nominally significant mouse E2 DMRs. Pathway analysis of genes associated with overlapping loci using the g.Profiler analysis suite28 identified a single significant GO category (GO: 0010646, frequency observed=0.19, expected=0.024, P=0.046) for ‘regulation of cell communication’ and an enrichment of SP-1 (M00196_4, frequency observed=0.51, expected=0.021, P=0.0084) and AP-2 (M00800_3, frequency observed=0.54, expected=0.021, P=0.0029) transcription factor-binding motifs.
