H4 (Ac-H4), associated with transcriptionally active euchromatin (10), and 3me-H3K27, associated with transcriptional repression (5,10,11), were highlighted in this study. The DNA methylation site- 5-methyl cytosine (5-MeC), DNA methylation binding protein (MBD1), and DNA methyltransferase (DNMT) 1 and 3 mediating DNA methylation (2,23) were analyzed. To further examine the function of DNA methylation during NSC differentiation, treatment with the DNA methylation inhibitor of, 5-aza-cytidine (AZA) was also studied.
