In addition, as illustrated in Fig. 2C, MDA+HAE levels increased in both the WT and Cyp2e1-null groups fed HFD compared to their LFD corresponding groups; however the increased levels of lipid peroxidation were also significantly higher in the WT-HFD compared with the null-HFD (Fig. 2C). Furthermore, Oxyblot analysis demonstrated that the levels of oxidized proteins (Fig. 2D upper panel) were markedly increased in the WT-HFD groups compared to those of the other three groups. The specificity of the oxidized protein bands was confirmed by using 1 derivatization Control Solution (Fig. 2D, lower panel) where no bands were detected. Immunoblot analysis revealed that the levels of 3-NT proteins, as a marker for nitrosative stress, were prominently higher in the WT-HFD group compared to those of the other three groups (Fig. 2E, upper panel), while p38 bands used as the loading control (Fig. 2B, lower panel) were similar in all groups.
