1×106 HDF51iPS cells were harvested by Accutase treatment (Life Technologies, Inc.), re-suspended in a 1:1 mixture of DMEM/F12 and Matrigel (BD Biosciences) and injected into the right testis of a C.B-17-Prkdcscid mouse (Charles River). Six to eight weeks after injection, tumors were dissected, fixed in 4%PFA, sectioned and stained with hematoxylin and eosin.
