DGGE is a rapid, commonly used method for mutation detection. The technology is based on the mobility of double-stranded DNA in polyacrylamide gel containing linearly increasing concentrations of denaturing chemicals.179,180 SSCP is a simple PCR-based mutation detection method. The mobility of double-stranded PCR fragments depends on the size of the DNA, since the polymorphisms might result in the altered mobility of single-stranded DNA by changing its conformation (Figure 6). The PCR products should be denatured by heat and formamide, followed by neutral polyacrylamide gel electrophoresis.181,182
