We designed 55 PCR amplimeres with 1kb overlaps (average amplimere length 7908 bases, range 6967–8362). The region spanned ~382.3kb (chr2:185163292-185545280, NCBI b36) (Figure 1). We were not successful in amplifying 5 of the amplimeres, representing a total of ~8% of the target sequence (29,341 bases).
