a bioinformatic assay that uses global gene expression profiles to bench mark unknown iPSCs with bona fide pluripotent cells44, was performed. The results of the PluriTest confirmed that the 12 iPSCs were highly similar in their transcriptome to previously described pluripotent stem cells (Sup. Fig. 4). Finally, pluripotency was directly assessed by determining the ability of all 12 iPSCs lines to form teratomas in nude mice. Upon sectioning and H&E staining, teratomas from all 12 iPSCs were found to contain representative tissue of all three (ectoderm, mesoderm, endoderm) germ layers (Sup. Fig. 5). Collectively, these data demonstrate successful generation of 12 high-quality, independent iPSC lines (picked from unique primary iPSC clones) from a family with BD.
