buffer (10 μl), custom paired end (PE) primer 1 (1 μl), custom PE primer 2 (1 μl), dNTP (deoxynucleotide triphosphate) mix (1.5 μl), Phusion polymerase (0.5 μl). The cleanup after amplification was done at 1:0.85 (AMPure beads/DNA). The concentration of each library was determined by real-time PCR using Agilent qPCR Library Quantification Kit and a MX3005P instrument (Agilent). Sequencing was performed on an Illumina HiSeq 2000 using 50–base pair paired-end reads.
