To start an efficient reprogramming, an appropriate amount of cells, in this case keratinocytes, is needed. Therefore, it is necessary to obtain a certain expertise in culturing keratinocytes from plucked hair. This has shown to be a drawback for the use of this cell type in various labs. The most important part is plucking the hair with an adequate root, including that the root has to be plucked with an intact ORS (Figure 3). The ORS is visible as a white covering around the root. Once plucked, hair can be stored in normal DMEM medium for several days at room temperature. This implies that hair can be shipped easily from all over the world without fearing the loss of the ability of keratinocytes to proliferate. The convenience for all the involved parties is a huge advantage of this method. The donors do not have to suffer from clinical and surgery investigations, they do not even have to go to hospital. The scientists can obtain the hair at any place with only little effort of the person plucking the hair. Compared
