Chunk #3 — MATERIALS AND METHODS — Targeted sequencing of CHRNA5

Source

Rare, low frequency and common coding variants in CHRNA5 and their contribution to nicotine dependence in European and African Americans.

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The Center for Inherited Disease Research (CIDR) performed next-generation targeted sequencing on CHRNA5. Details of the sequencing procedures and quality control measures are provided in the Supplementary Methods. The mean on-target coverage was 180X, and greater than 96% of on-target bases had a depth greater than 20X.