Fibroblast cell lines were generated from skin punch biopsies donated by participants enrolled in a study of the effects of alcohol in social drinkers (9 males) (Milivojevic et al., 2014) or in a clinical trial for heavy drinkers (10 males and 2 females) (Kranzler et al., 2014) at the University of Connecticut Health Center (UCHC). All fibroblast samples were from Caucasian donors. Fibroblasts were reprogrammed to pluripotency by the UCHC Stem Cell Core using retrovirus constructs expressing five factors (OCT4, SOX2, KLF4, c-MYC, and LIN28) for 34 lines or sendai virus constructs expressing four factors (OCT4, SOX2, KLF4, c-MYC) for 2 lines. 2 to 4 weeks after viral transduction, individual iPSC colonies were identified by morphological characteristics and selected clones were expanded and cultured as separate cell lines in human embryonic stem cell media on a feeder layer of irradiated mouse embryonic fibroblasts.
