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Chunk #9 — Materials & Methods — Paired pulse ratio

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Chronic ethanol and withdrawal effects on kainate receptor-mediated excitatory neurotransmission in the rat basolateral amygdala.
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Paired-pulse facilitation (PP) during whole-cell recordings was measured using pairs of electrical stimuli of equal intensity at 25, 50, or 250 msec interpulse intervals. Ratios of the amplitudes of the evoked EPSCs were calculated as the amplitude of the second event minus the amplitude of the first event divided by the amplitude of the first event. All values were expressed as mean ± SEM, and data were subjected to a one way ANOVA and Newman-Kuels post-test with P<0.05 considered statistically significant.