On the session day, subjects completed a urine toxicology screen, alcohol breathalyzer test, and urine pregnancy test; positive finding/s resulted in termination of their study participation. Subjects reported to the laboratory fasting and then consumed a calorie-restricted meal to control for possible dietary effects on alcohol absorption. Subjects were in a quiet laboratory room, where they were monitored by the research nurse. A cumulative dosing procedure was used. At time 0, a placebo drink was administered. Three equal doses of alcoholized beverage were then administered at 45 min intervals (+45, +90 and +135); subjects had 10 minutes to finish each drink. Subjects completed subjective response assessments using a laptop computer and mouse at baseline (−25 min), following each drink (+15, +60, +105, +150 min) and at 45-minute intervals for the remainder of the session (+195, +240, +285 min) (Figure 1). Following completion of the laboratory session, subjects were admitted to the inpatient Johns Hopkins General Clinical Research Unit to monitor them while intoxicated and were discharged the following morning.
