from T55 (Figure 5A). FACS analysis using erythroblast markers (CD45− CD33 − glycophorin A [GPA]+, transferrin [CD71]+) confirmed the reduced erythroid potential of T42 iPSC lines compared with T55 (Figures 5B and 5C). To evaluate the functionality of these cells, we plated them into methylcellulose and measured their erythroid colony-forming potential. iPSC lines derived from donor T42 yielded fewer erythroid colonies than those from T55 (Figure 5D). These results demonstrate that iPSC lines derived from different donors can possess significant variability in lineage commitment potential irrespective of their cell source.
