Statistical significance was determined using Student t test or ordinary one-way ANOVA with Tukey’s multiple comparison post-test to compare the means of multiple groups. Data are shown as mean ± SEM and were considered statistically significant at p < 0.05. For the two-bottle choice test analysis, we used the two-way analysis of variance (ANOVA) with post hoc Bonferroni with GraphPad Prism 7.0c (GraphPad Software Inc., La Jolla, CA, USA).
