Chunk #8 — Methods — Quantitative real-time PCR (qPCR)

Source

Integrative transcriptome network analysis of iPSC-derived neurons from schizophrenia and schizoaffective disorder patients with 22q11.2 deletion.

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qPCR was carried out on reverse transcribed cDNA from the same RNA samples used for the RNA-seq by the 2-ΔΔCt method as previously described [39]. A detailed description and the primers used for this analysis can be found in Additional file 2.