Whole transcriptome libraries were prepared using total RNAs from 15 control and 14 alcoholic postmortem prefrontal cortices and commercial RNAs following the SOLiD™ Total RNA-Seq Kit (ABI, Carlsbad, CA). The rRNA depleted RNAs were fragmented, and adaptors were ligated to their ends; cDNAs were then synthesized. The gene specific and whole transcriptome libraries were sequenced using the ABI SOLiD system.
